SELF-SUFFICIENCY OF NATURAL E. COLI POLYSOMES FOR AMINO ACID INCORPORATION
نویسندگان
چکیده
منابع مشابه
Amino Acid Incorporation into Proteins by Escherichia Coli Ribosomes.
Evidence that ribosomes are an important site of protein synthesis has come mainly from experiments with animal cells. It has been shown in vivo that C14 amino acids are incorporated into peptide linkage first in ribosomes, and by addition of cold amino acids, they can be chased to the bulk of the proteins of the cell.1-3 In vitro, they become bound in ribosomal proteins2 or, after their passag...
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Amino acids have been extensively used in several processes of the pharmaceutical and food industries. Treatments for the recovery and reuse of the wastewaters generated from these processes are few and little known. This work aims at studying the influence of different variables on the sorption of L-cysteine, contained in aqueous solutions, on clinoptilolite mineral. L-Cysteine (Cys) was s...
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Unnatural amino acid (UAA) incorporation by amber codon suppression offers scientists a powerful tool to modify the properties of proteins at will. UAA incorporation has been used for a plethora of fundamental research applications and, more recently, also for the selective modification of therapeutic proteins. In this review most recent developments in Escherichia coli codon expansion and, unn...
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Although much has been learned in recent years concerning amino acid incorporation into protein, the mechanism of the process has remained obscure. This is due partly to the complexity of the problem and partly to the lack of experimental systems sufficiently simple and free from extraneous reactions so that mechanism studies can be performed with confidence. The experiments of Littlefield and ...
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13 E. W. Davie, V. V. Koningsberger, and F. Lipmann, Arch. Biochem. and Biophys., 65, 21, 1956. 14 V. V. Koningsberger, A. M. van de Ven, and J. T. G. Overbeck, Royal Dutch Acad. Sci., Ser. B 60, 141, 1957. 16 R. S. Schweet, R. W. Holley, and E. H. Allen, Arch. Biochem. and Biophys., 71, 311, 1957. 16 M. B. Hoagland, P. C. Zamecnik, and M. L. Stephenson, Biochim. et Biophys. Acta, 24, 215, 1957...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1964
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.52.5.1283